Fig 4. 3CD expression causes redistribution of Arf1, GBF1, and PI4KB to sites of PI4P synthesis without affecting Golgi or ER integrity.

(A) HeLa cells expressing 3CD were immunostained for the presence of the following: (i) Arf1, (ii) GBF1, or (iii) PI4KB (green). 3CD expression was monitored (red); the nucleus is stained witth DAPI (blue). (B) The same experiment as performed in panel A, with immunostaining for PI4P (red) instead of 3CD. (C) HeLa cells expressing 3CD were immunostained for the presence of the following: (i) Giantin or (ii) Calnexin (green). 3CD expression was monitored (red); the nucleus is stained witth DAPI (blue).