Figure 1.

Label-free quantitative MS identification of a set of human proteins that associate with PL. (A) Length distribution of the poly-L-lysine peptide mix used to elute proteins interacting with poly-L-lysine agarose, as determined by mass spectrometry. (B) Distribution of the identified PL interactors according to their enrichment during the PL-agarose pulldown (PD) from the cytosolic extracts of 293T cells. A total of five independent biological experiments. (C) Distribution of the identified interactors plotted against their abundance in cytosol (summed intensities). The subset of the 309 highest-enriched proteins (mean plus 1 standard deviation) is labeled red and is considered as the interactome of PL in this study.