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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Immunol Regen Med. 2018 Feb 22;1:1–12. doi: 10.1016/j.regen.2018.01.003

Figure 3. IL-33 increases local population of CD4+ Foxp3+ Tregs in blank scaffold implant.

Figure 3

(A) Representative flow cytometry plots and quantification of percentages and overall numbers of live CD3+ CD4+ Foxp3+ Tregs isolated from control and IL-33 scaffolds seven days after implant. Frequency of ST2+ Foxp3+ CD4+ T cells seven days after implant as quantified by flow cytometry (B) Representative flow cytometry plot and quantification of percentages and overall numbers of ST2+ Foxp3+ CD4+ T cells seven days after implant (C) Representative flow cytometry plots and quantification of percentages of CD4+ Foxp3+ cells and ST2+ Tregs isolated from spleens of animals receiving control or IL-33 scaffolds. Overall numbers are normalized to weight of tissue immediately after isolation. Graphs depict mean ± SEM. N = 5-6 scaffolds per condition. **p < 0.01 ***p < 0.001 ****p < 0.0001 Statistics determined by unpaired t-test. (1.5 column)