Figure 7.

miR-142-5p targets PTEN in MDA-MB-231 cells. (A) The binding site between miR-142-5p and PTEN 3′UTR and the mutant position of PTEN 3′UTR. (B) Similar levels of luciferase activity were observed in the cells harboring the mutant 3′UTR and the WT/NC control cells. Compared with the miR-NC inhibitor, transfection of miR-142-5p inhibitor and PTEN-3′UTR-WT vector but not the PTEN-3′UTR-mutant vector induced the luciferase activity in MDA-MB-231 cells. **P<0.01 vs. miR-NC inhibitor. miR, microRNA; PTEN, phosphatase and tensin homolog; NC, negative control; UTR, untranslated region; WT, wild type.