Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 28;17(6):7645–7651. doi: 10.3892/mmr.2018.8806

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 1. — TIP5 interacts with TCF7L24. (A) A GST pull-down assay was performed to examine the interaction between GST-TCF7L24 fusion protein and endogenously expressed TIP5 in 7404 cells. (B) An immunoprecipitation assay was performed to examine the interaction between exogenously expressed TIP5 (myc-TIP5) and TCF7L24 (Flag-TCF7L24) in 7404 cells. (C) An immunoprecipitation assay was performed to examine the interaction between endogenously expressed TIP5 and TCF7L24 in 7404 cells. (D) A topflash reporter assay was performed in 7404 cells. (E) An immunoprecipitation assay was performed to examine the effects of TIP5 on the interaction between exogenously expressed β-catenin (HA-β-catenin) and TCF7L24 (Flag-TCF7L24) in 7404 cells. Data are presented as the mean ± standard deviation. sh, small hairpin; GST, glutathione S-transferase; TCF7L24, Transcription factor 4; TIP5, transcription termination factor-1 interacting protein 5; con, control; IP, immunoprecipitation; HA, hemagglutinin tag; input, cell lysate. ^##P<0.01, **P<0.01 vs. the respective sh con group.