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. 2018 Mar 28;17(6):7645–7651. doi: 10.3892/mmr.2018.8806

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — TIP5 promotes the proliferation and migration of HCC cells. (A) The overexpression of TIP5 (myc-TIP5) in 7404 and Huh7 cells was identified. (B) An MTT assay was performed to examine the effects of TIP5 on the proliferation of HCC cells. (C) A Boyden chamber assay was performed to examine the effects of TIP5 on the migration of HCC cells. Magnification, ×10. (D) A soft agar assay was performed to examine the effects of TIP5 on the anchorage-independent growth of HCC cells. (E) TIP5 induced a morphological change in 7404 cells. Data are presented as the mean ± standard deviation. Magnification, ×10. *P<0.05, **P<0.01 vs. pcDNA3.1. HCC, hepatocellular carcinoma; TIP5, transcription termination factor-1 interacting protein 5; OD, optical density.