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. 2018 Mar 28;17(6):7645–7651. doi: 10.3892/mmr.2018.8806

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — TIP5 knockdown inhibits the growth and migration of HCC cells. (A) The effects of TIP5 knockdown in 7404 and Huh7 cells was assessed using western blot analysis. (B) An MTT assay was performed to examine the effects of TIP5 knockdown on the growth of HCC cells. *P<0.05 vs. sh TIP5 (1#) or (2#). Data were presented as the mean ± standard deviation. (C) A Boyden chamber assay was performed to examine the effects of TIP5 knockdown on the migration of HCC cells. Magnification, ×10. (D) A soft agar assay was performed to examine the effects of TIP5 knockdown on the anchorage-independent growth of HCC cells. Magnification, ×10. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. sh con. HCC, hepatocellular carcinoma; TIP5, transcription termination factor-1 interacting protein 5; sh, small hairpin; con, control; OD, optical density.