Figure 3.

Form regulates miR-149 and EphB3 expression and suppresses AKT/PI3K expression and STAT3 signaling in SW1116 and HCT116 cells. (A) SW1116, (B) HCT116 and (C) NCM460 cells were treated with Form (20, 50, and 100 µM) for 48 h, then miR-149 and EphB3 mRNA were determined using reverse transcription-quantitative polymerase chain reaction. Data are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01 vs. control, n=5. (D) SW1116 and HCT116 cells were incubated with Form (100 µM) for 12 h, and the protein levels of p- and total PI3K, AKT, ERK, and STAT3 were determined using western blotting. The band intensities relative to AKT, PI3K, ERK and STAT3 are presented as the mean ± standard deviation. *P<0.05 vs. the control; n=6. Form, Formononetin; p-PI3K, phosphorylated-phosphoinositide 3 kinase; AKT, protein kinase B; ERK, extracellular regulated signal kinase; STAT3, signal transducer and activator of transcription 3; EphB3, Ephrin type-B receptor 3.