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. 2018 Apr 5;17(6):7721–7729. doi: 10.3892/mmr.2018.8857

Figure 4.

Figure 4.

Both the mimic miR-149 and siEphB3 enhance Form-induced inhibition of proliferation of colon cancer cells. (A) RT-qPCR analysis of miR-149 in SW1116 and HCT116 cells transfected with mimic miR-149 or negative control. Data are depicted as the mean ± standard deviation. **P<0.01 vs. control, n=5. (B) Western blot analysis for EphB3 expression detection in SW1116 and HCT116 cells transfected with mimic miR-149. (C) RT-qPCR for siRNA-mediated silencing verification of EphB3 mRNA in SW1116 and HCT116 cells transfected with siEphB3 or siRNA control. *P<0.05 vs. control, n=5. SW1116 and HCT116 cells transfected with (D) mimic-NC or mimic miR-149 for 24 h or transfected with (E) siEphB3 or siNS for 24 h. Transfected cells were then treated with 100 µM Form for 24 h. Cell viability was determined using the MTT assay. Data are illustrated as the mean ± standard deviation, *P<0.05 and **P<0.01 vs. control, n=5. (F) Transwell assay demonstrated that miR-149 overexpression and (G) EphB3 downregulation enhanced Form-inhibited cell invasion in HCT116 cells (magnification, ×400). Data are presented as the mean ± standard deviation, *P<0.05 and **P<0.01 vs. the control, n=5. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; si, small interfering; miR, microRNA; NS, normal control; EphB3, Ephrin type-B receptor 3; Form, Formononetin.