Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus Musculus) | Gt(ROSA)26Sortm13(CAG-MYC,-CD2*)Rsky(referred as StopFLMYC) | The Jackson Laboratory (Bar Harbor, ME) |
MGI:5444670 | Maintained on a C57Bl/6 background |
| Strain, strain background (Mus Musculus) | Tg(Nes-cre)1Kln (referred as Nestin-cre) | The Jackson Laboratory | MGI:2176173 | Maintained on a C57Bl/6 background |
| Strain, strain background (Mus Musculus) | Foxg1tm1(cre)Skm(referred as Foxg1-cre) | The Jackson Laboratory | MGI:1932522 | Maintained on a C57Bl/6 background |
| Strain, strain background (Mus Musculus) | Myc-deficient mice (c-myc-/-) | Provided by Troy Baudino | Baudino et al. (2002) | Maintained on a C57Bl/6 background |
| Strain, strain background (Mus Musculus) | CD-1 IGS Mouse (referred as CD-1) | Charles River (Wilmington, MA) |
Strain code: 022 | Wildtype timed pregnant mice |
| Antibody | Rabbit anti-4E-BP1 | Cell Signaling (Danvers, MA) |
9644 | 1:1000 |
| Antibody | Mouse anti-5.8S ribosomal RNA [Y10B] | Abcam (United Kingdom) |
ab171119 | 1:50; antigen retrieval with steaming in citric acid |
| Antibody | Mouse anti-ACTB | Cell Signaling | 12262 | 1:2000 |
| Antibody | Rat anti-BrdU | Biorad (Hercules, CA) | MCA2060 | 1:200; antigen retrieval with steaming in citric acid |
| Antibody | Rabbit anti-MYC | Abcam | ab32072 | 1:100 for IHC, antigen retrieval with steaming in citric acid; 1:2000 for WB |
| Antibody | Rat anti-CTIP2 | Abcam | ab18465 | 1:200 |
| Antibody | Rabbit anti-CUX1 | Santa Cruz Biotechnology (Dallas, TX) |
sc13024 | 1:200 |
| Antibody | Mouse anti-EIF3η | Santa Cruz Biotechnology | sc137214 | 1:100; antigen retrieval with steaming in citric acid |
| Antibody | Mouse anti-Fibrillarin | Abcam | ab4566 | 1:250; antigen retrieval with steaming in citric acid |
| Antibody | Mouse anti-GAPDH | Cell Signaling | 97166 | 1:1000 |
| Antibody | Rabbit anti-p4E-BP1 | Cell Signaling | 2855 | 1:200 for IHC; 1:1000 for WB |
| Antibody | Rabbit anti-PAX6 | Biolegend (San Diego, CA) |
901301 | 1:100; antigen retrieval with steaming in citric acid; 1:1000 for FACS |
| Antibody | Rabbit anti-pS6 | Cell Signaling | 5364 | 1:200 for IHC; 1:1000 for WB |
| Antibody | Rabbit anti-pS6K | Cell Signaling | 9234 | 1:1000 |
| Antibody | Mouse anti-pVimentin | Enzo Bioscience (Farmingdale, NY) |
ADI-KAM-CC249-E | 1:400 |
| Antibody | Mouse anti-RPL10A | Novusbio (Littleton, CO) | H00004736-M01 | 1:500 |
| Antibody | Rabbit anti-RPL11 | Santa Cruz Biotechnology | sc50363 | 1:50 |
| Antibody | Rabbit anti-RPS12 | Proteintech (Chicago, IL) |
16490–1-AP | 1:50 |
| Antibody | Rabbit anti-S6 | Cell Signaling | 2217 | 1:1000 |
| Antibody | Rabbit anti-S6K | Cell Signaling | 9202 | 1:1000 |
| Antibody | Mouse anti-TUJ1 | Biolegend | 801202 | 1:100 for IHC; 1:1000 for FACS |
| Antibody | Rabbit anti-Vinculin | Cell Signaling | 13901 | 1:1000 |
| Recombinant DNA reagent | Quaser 570 coupled 5.8S pre-rRNA FISH probe | Provided by Debra Silver | 1:200 | |
| Recombinant DNA reagent | Quaser 670 coupled 5.8S total rRNA FISH probe | Provided by Debra Silver | 1:200 | |
| Commercial assay or kit | RecoverAll Total Nucleic Acid Isolation Kit for FFPE | Ambion (Foster City, CA) | AM1975 | Manufacturer's protocol |
| Commercial assay or kit | Ovation RNA-Seq System V2 | Nugen (San Carlos, CA) | 7102 | Manufacturer's protocol |
| Commercial assay or kit | Ovation Ultralow System V2 1–16 | Nugen | 0344 | Manufacturer's protocol |
| Commercial assay or kit | TruSeq RNA Library Prep Kit v2 | Illumina (San Diego, CA) | RS-122 | Manufacturer's protocol |
| Commercial assay or kit | Rneasy Micro Kit | Qiagen (Germany) | 74004 | Manufacturer's protocol |
| Commercial assay or kit | Pierce BCA Protein Assay Kit | Thermo Fisher Scientific (Waltham, MA) |
23227 | |
| Commercial assay or kit | Click-iT plus OPP protein synthesis assay kit | Thermo Fisher Scientific | C10456 | |
| Chemical compound, drug | O-propargyl-puromycin (OPP) | Life Technologies (Carlsbad, CA) |
C10459 | IP injection, dosage: 50 mg/kg |
| Chemical compound, drug | KJ-Pyr-9 | Tocris (United Kingdom) | 5306 | IP injection, dosage: 10 mg/kg |
| Chemical compound, drug | 35S-Methionine | Perkin Elmer (Waltham, MA) |
NEG709A | 51 μCi |
| Chemical compound, drug | 5-Bromo-2′-deoxyuridine (BrdU) | Sigma (St. Louis, MO) | B5002 | IP injection, 50 mg/kg |
| Software, algorithm | TopHat | https://ccb.jhu.edu/software/tophat/index.shtml | v2 | RNAseq analysis |
| Software, algorithm | Cufflinks | http://cole-trapnell-lab.github.io/cufflinks/ | v2 | RNAseq analysis |
| Software, algorithm | DAVID | https://david.ncifcrf.gov/ | v6.7, 6.8 | RNAseq analysis |
| Software, algorithm | GSEA | http://software.broadinstitute.org/gsea/index.jsp | v2 | RNAseq analysis |
| Software, algorithm | R Studio | Rstudio, Inc. | v0.99 | RNAseq analysis |
| Software, algorithm | Prism | GraphPad | v7 | Statistical analysis |
| Software, algorithm | FIJI (Image J) | https://fiji.sc/# | v1 | Image analysis |
| Software, algorithm | Imaris | Bitplane | Image analysis |