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. Author manuscript; available in PMC: 2018 Jul 3.
Published in final edited form as: Nature. 2018 Jan 3;553(7688):291–294. doi: 10.1038/nature25178

Figure 4. ptsT enables enhanced trehalose metabolism.

Figure 4

a, Structure of horizontally acquired trehalose metabolism module found in RT078 and closely related strains. b, Deletion of the trehalose transporter from a clinical RT078 strain (CD1015) ablates its ability to grow on 10 mM trehalose. Expression of ptsT from an inducible plasmid restores growth of CD1015ΔptsT on 10 mM trehalose, (CD1015, n=4; CD1015ΔptsT, n=4; CD1015ΔptsT::ptsT, n=5). c, Expressing ptsT from an inducible plasmid enables enhanced growth of CD630 (RT012) on 10 mM trehalose (CD630 n=3; CD630::ptsT n=3). d, The ptsT provides a competitive advantage in complex microbial communities. Dashed grey line (CI = 1) indicates equal fitness of the competing strains, points above this line represent out-competition by CD1015. All points (Fig 4b-d) represent biologically independent samples, bars are mean, p values derived from t-test (2-tailed) and Holm corrected for multiple comparisons where appropriate.