Table 2.
Acquisition parameters and observed spectral features for 2D heteronuclear correlation experiments performed on 13C/15N Surf6-N.
| Experiment | 1H,15N-HSQC | N_HNINEPT | 13C-CON | 15N-CON |
|---|---|---|---|---|
| Points (F2 x F1) | 2048 x 510 | 2048 x 154 | 1024 x 510 | 2048 x 104 |
| Acquisition time (ms) | 113 x 92 | 369 x 21 | 236 x 92 | 369 x 24 |
| Scans | 16 | 48 | 32 | 296 |
| Recycle Delay (s) | 1 | 1 | 1 | 1 |
| Exp Time (hrs:min) | 2:40 | 2:54 | 12:22 | 12:25 |
| <FWHH 1HN> (Hz) | 26.1 ± 2.0 (n = 35) | 30.7 ± 2.1 (n = 31) | -- | -- |
| <FWHH 13C′> (Hz) | -- | -- | 7.5 ± 0.8 (n = 34) | 20.9 ± 0.8 (n = 34) |
| <FWHH 15NH> (Hz) | 9.7 ± 1.5 (n = 35) | 7.3 ± 1.6 (n = 31) | 9.7 ± 1.5 (n = 34) | 5.8 ± 1.0 (n = 34) |
| Signal-to-noise | 923 | 143 | 142 | 80 |
| # peaks (observed/possible) | 142/177 | 155/177 | 161/185 | 162/185 |