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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: J Thromb Haemost. 2018 May 8;16(6):1211–1225. doi: 10.1111/jth.14005

Figure 1. GSK3β Inhibition does not rescue 2MeSADP-induced platelet aggregation and thromboxane generation in human and murine platelets.

Figure 1

A) Washed human and murine (e.g. mouse) platelets were pre-treated with the DMSO (vehicle control), BX-795 (1 μM), aspirin (10 μM) or indomethacin (10 μM) at 37°C for 5 min followed by stimulation with 2MeSADP (50 nM) under stirred conditions. Platelet aggregation was measured by aggregometry. The tracings are representative of data from at least three independent experiments. B) Washed human and murine platelets were pre-treated with DMSO (vehicle control), the PDK1 inhibitor BX-795 (1 μM) or the GSK3β inhibitor SB-216763 (5 μM) or both for 5 min followed by stimulation with 2MeSADP (50 nM) under stirred conditions for 3.5 min at 37°C. Platelet aggregation was measured by aggregometry. The tracings are representative of data from at least three independent experiments. C) Thromboxane generation was measured as described in the experimental section. Graphs represent mean ± SD from at least three independent experiments.