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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: J Thromb Haemost. 2018 May 8;16(6):1211–1225. doi: 10.1111/jth.14005

Figure 4. Role of p38 Kinase in 2MeSADP induced platelet activation.

Figure 4

(A) Washed human platelets were incubated BX-795 (1 μM), SB-203580 (5 μM), or DMSO (vehicle control) followed by stimulation with 2MeSADP (2.5 nM) under stirred conditions for 5 minutes. Platelet proteins were separated using SDS-PAGE and probed for phosphorylation of p38 (Thr180/Tyr182), ERK1/2 (Thr202/Tyr204), and cPLA2 (S505). (B) Washed human platelets were incubated with SB-203580 (5 μM) followed by stimulation with 2MeSADP at various concentrations under stirred conditions for 5 minutes. Platelet proteins were separated using SDS-PAGE and probed for phosphorylation of p38 (Thr180/Tyr182). Actin is loading control. Representative of n≥3 independent experiments. (C, D) Proposed models for ADP-induced activation of cPLA2 and p38.