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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Hear Res. 2018 Mar 13;364:129–141. doi: 10.1016/j.heares.2018.03.014

Figure 3.

Figure 3

Representative radial sections (3 μm) of cochlear organotypic cultures stained with toluidine blue. (A) Control (0 μM) sample cultured for 24 h showing three rows of outer hair cells (OHC), one row of inner hair cells (IHC), Deiters cells (DC, green arrow), inner and outer pillar cells (PC, pink arrow), inner sulcus cells (ISC, white arrow), basilar membrane (dashed line) and tympanic border cells (TBC) beneath the basilar membrane. (B) Section from culture treated for 6 h with 200 μM PQ. OHC and IHC still present, missing or severely shrunken TBC indicated by arrowhead beneath the basilar membrane. Note pale cytoplasm in remaining TBC and shrunken DC with pale cytoplasm. (C) Section from culture treated for 12 h with 200 μM PQ. IHC and OHC still present; missing DC labeled with *; many missing or severely shrunken TBC (arrowhead). (D) Section from culture treated with 200 μM PQ for 24 h. Remaining IHC and OHC are pale and shrunken. Note missing DC (*) and TBC (arrowhead).