Effect of MeHg on proliferation. HTR8/SV-neo cells were treated with 0, 0.01, 0.1, and 1 μg/mL MeHg and the total number of cells was counted at 0, 24, 48, and 72 hours (N=3 replicates x 2 duplicates). At 24, 48, and 72 hours, the number of cells in the 1 μg/mL MeHg treatment group was significantly less than the number of cells in the 0 μg/mL group (*** denotes p<0.001). Cell number was not affected by 0.01 or 0.1 μg/mL MeHg at any time point.