Effect of MeHg on sftpd expression. HTR8/SV-neo cells were treated with 0, 0.01, 0.1, or 1 μg/mL MeHg for 24 hours (N=3 replicates x 2 duplicates). Quantification of sftpd mRNA was carried out by RT-PCR. Relative to control expression (horizontal line), 0.01 μg/mL MeHg caused a slight decrease in sftpd expression (* denotes p<0.05) while 1 μg/mL MeHg caused a dramatic increase (**** denotes p<0.0001). Expression increased slightly with 0.1 μg/mL MeHg, but it was not statistically significant.