Table 1.

Oxidative stress gene array results. HTR8/SV-neo cells were treated with either 0 μg/mL or 1 μg/mL MeHg for 24 hours (N=3 replicates x 1 duplicate). Fold-change in expression for each gene was determined using the ΔΔCt method relative to the endogenous control genes (hmbs, pgk1, and ppia) and the control treatment of 0 μg/mL. The genes below are grouped by function, and significant values (p<0.05) are shown in bold.

Gene	2−ΔΔCt	SEM	t	p	Gene	2−ΔΔCt	SEM	t	p	Gene	2−ΔΔCt	SEM	t	p
REGULATORY					gsr	1.116	0.134	0.864	0.479	oxsr1	0.874	0.105	−1.196	0.354
Protein Degradation					gpx7	0.810	0.097	−1.957	0.190	sgk2	0.335	0.040	−16.522	0.004
sels	2.518	0.302	5.023	0.037	gpx1	0.463	0.056	−9.679	0.011	stk25	0.081	0.010	−95.054	0.0001
rnf7	0.989	0.119	−0.094	0.934	Peroxidases					Oxidases/Oxidase Activators
Cell Cycle Regulation					prdx4	2.572	0.309	5.093	0.037	cyba	2.439	0.293	4.916	0.039
gft2i	0.815	0.098	−1.887	0.200	prdx3	2.115	0.254	4.393	0.048	aox1	2.230	0.268	4.595	0.044
Transcription Regulation					prdx6	1.539	0.185	2.918	0.100	ncf2	1.672	0.201	3.350	0.078
foxm1	1.220	0.146	1.500	0.2724	prdx2	1.484	0.178	2.718	0.113	nox5	1.320	0.158	2.018	0.181
Translation Regulation					prdx1	1.432	0.172	2.515	0.128	Hydrolases
csde1	1.050	0.126	0.393	0.7323	prdx5	1.134	0.136	0.982	0.430	ephx2	2.297	0.276	4.704	0.042
Pro-Apoptotic					pxdn	0.940	0.113	−0.534	0.688	nudt1	1.386	0.166	2.320	0.146
bnip3	1.485	0.178	2.720	0.1128	ptgs2	0.826	0.099	−1.756	0.221	Phosphatases
CELL SIGNALING					ptgs1	0.684	0.082	−3.849	0.061	dusp1	1.124	0.135	0.918	0.456
Cell Adhesion					Reductases					Deacetylases
pdlim2	1.434	0.172	2.522	0.1278	msra	1.211	0.145	1.453	0.283	sirt2	0.211	0.025	−31.074	0.001
Cellular Signaling					oxr1	1.090	0.131	0.691	0.561	OTHER
ccl5	2.119	0.254	4.400	0.0480	dhcr24	1.066	0.128	0.517	0.657	Copper Binding
nos2	1.670	0.200	3.342	0.0791	srxn1	0.390	0.047	−13.036	0.006	prnp	1.879	0.225	3.898	0.060
gpr156	1.579	0.190	3.056	0.0925	txnrd2	0.136	0.016	−52.904	0.001	atox1	1.057	0.127	0.447	0.699
prex1	0.911	0.109	−0.811	0.5025	txrnd1	0.094	0.011	−80.621	0.001	ccs	0.759	0.091	−2.646	0.118
ENZYMATIC					Free Radical Detoxifiers					Metallothionein
Glutathione Pathway					scara3	2.127	0.255	4.415	0.048	mtl5	1.127	0.135	0.936	0.448
mgst3	2.040	0.245	4.248	0.051	cat	1.178	0.141	1.261	0.335	Catabolism of Lipoproteins
glrx2	1.994	0.239	4.153	0.053	mpv17	0.821	0.098	−1.822	0.210	apoe	1.257	0.151	1.702	0.231
gstz1	1.889	0.227	3.921	0.059	sod2	0.098	0.012	−76.823	0.0002	Extracellular Antioxidant
gss	1.522	0.183	2.859	0.104	sod1	0.043	0.005	−185.715	0.0001	sepp1	0.252	0.030	−24.791	0.002
gpx4	1.303	0.156	1.938	0.192	Kinases					Immune Function
gpx3	1.229	0.147	1.553	0.261	pnkp	1.034	0.124	0.276	0.809	sftpd	7.052	0.846	7.151	0.019