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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Bioelectrochemistry. 2018 Mar 29;122:123–133. doi: 10.1016/j.bioelechem.2018.03.014

Figure 6.

Figure 6

Effect of the amplitude of the opposite polarity phase of nsEP on electroporation efficiency. Pr uptake was monitored in VCM exposed to trains of 10 uni- or bipolar nsEP, 5Hz, starting at 28 s. The pulse width was 830 ns or 830+830 ns (A and B), and 200 ns or 200+200 ns (C). Pulse amplitude of 800 V translates into 30 kV/cm at the cell location, and smaller amplitudes produce proportionally smaller fields. The configuration of each pulse and the amplitudes of the first/second phases are shown next to the plots. The amplitude of the first phase was kept constant at 400V (A) or at 800V (C) while varying the second phase. In panel B, we specifically compared bipolar pulses with the same spectral content, 400V/800V and 800V/400V, and compared them with respective unipolar pulses; see text for more details. Mean+/− s.e., 15-20 cell per group. Non-overlapping error bars indicate statistically significant difference at p<0.05 or better.