Figure 6.

CC131 growth (A) and L-lactate production (B) and TK.032 growth (C) and L-lysine production (D) were measured in photobioreactors to stationary phase. Subsequently, light limitation was verified by transfer of culture to flasks in which I_INSV⁻¹ was significantly higher. Samples were obtained for cell density measurement approximately every 24 hours and L-lactate or L-lysine concentration was determined. The growth retardation phase model was fit to the first 14 days of growth and the first 5 days of L-lactate or L-lysine production after which values deviated significantly from predictions (dotted line). The initial rate method was used to determine η and ρ and then m_ν was determined using the best fit of Equations 17 and 18. After ~21 days (vertical dashed line), samples from each photobioreactor were transferred to shake flasks. η and ρ were determined for this new growth condition by a best fit of Equations 17 and 18 using the value of m_ν determined in the photobioreactors. Parameter values are given in Table 4. Error bars represent the standard error of at least two biological replicates. Non-visible error bars are smaller than the data symbol.