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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Metab Eng. 2018 Mar 27;47:230–242. doi: 10.1016/j.ymben.2018.03.017

Table 3.

Photosynthetic Efficiency Under Various Experimental Conditions

Vessel Osmolarity (mM) T (°C) S/V (m−1) IINa (IINSV−1)b LGRc η
Synechococcus PCC7002
 Flask (25 mL)e 720 37 201 180 130000 62±3 0.23±0.03
 Tube 720 38 106 270 100000 51±6 0.25±0.04
 Flask (45 mL) e 720 37 120 180 78000 41±3 0.26±0.03
 Tube 720 38 106 200 77000 38±2 0.25±0.03
 Tube 720 38 106 140 54000 28±2 0.26±0.03
 Tube 720 38 106 66 25000 15±0.8 0.29±0.03
 Tube 720 38 106 23 8600 4.5±0.2 0.26±0.03
Lower Temperature
 Tube 720 30 106 270 100000 63±3 0.31±0.03
 Tube 720 30 106 66 25000 12±0.8 0.24±0.03
Higher Salinity
 Bottle (MMA) 1000 30 40 330g 47000 17±0.9 0.18±0.01
 Bottle (MMA) 1000 30 40 180g 26000 10±0.2 0.20±0.01
 Tube (2× NaCl) 1300 38 106 270 100000 46±3 0.22±0.03
 Tube (4× NaCl) 2600 38 106 270 100000 22±3 0.11±0.02
Synechococcus elongatus UTEX2973
 Flask (25 mL) e 38 37 201 180 130000 95±4 0.36±0.04
 Tube 38 38 106 220 84000 74±3 0.44±0.05
 Flask (50 mL) e 38 37 110 180 72000 62±4 0.43±0.05
 Tube 38 38 106 180 70000 58±3 0.41±0.05
 Tube 38 38 106 150 56000 41±2 0.37±0.04
 Flask (100 mL) e 38 37 64 180 42000 25±4 0.29±0.05
 Tube 38 38 106 75 29000 23±1 0.40±0.04
Higher Salinity
 Flask (1× NaCl)e,f 346 37 100 180 72000 23±0.9 0.16±0.02
 Flask (2× NaCl)e,f 654 37 100 180 72000 0 0
Vessel Osmolarity (mM) T (°C) S/V (m−1) IIN Ref. Yieldd Inferred η
Synechococcus PCC7002 - High Light
 Tube 720 38 NA ~2000g (Xiong et al., 2015) NA ~0.04h
a

[μmol photons m−2 s−1 Standard error is 10% of measured value. Light provided by 4000K Cool White LED light unless otherwise specified.

b

[μmol photons L−1 hr−1]

c

[mg DW−1 L hr−1]

d

[g DW (mol photons)−1]

e

Flask experiments performed in 250 mL shake flasks with varying volume.

f

UTEX2973 experiments with BG11 supplemented with specified Media A equivalents of NaCl.

g

Halogen light.

h

Experiment from Xiong, et al. found an 80% decrease in linear growth rate when IIN was increased from 50 umol m−2 s−1 to 2000 umol m−2 s−1 (Xiong et al., 2015). This was used to estimate η under very high light intensity from the experiments performed in this work. Error values are standard error of the mean propagated from LGR and IIN measurements of at least three biological replicates. Bold text to emphasize variables of interest for each experiment. MMA is Modified Media A (Table 1).