Fig. 1.

JEV is able to infect the cells forming the BBB. Confluent monocultures of HBECs and human astrocytes were infected with JEV and fixed at 3 days post infection (dpi) at MOI = 1. Using electron microscopy, JEV particles (arrows) were detected in cytoplasmic vacuoles in both HBECs (A–B) and human astrocytes (C–D); most were hexagonal in shape and 40–50 nm diameter with an electron dense core and an outer membrane (scale bar = 100 nm).