Table 3.

Genetic diversity and evolutionary dynamics of the two haplogroups (A and B) identified from cox1 sequences of R. appendiculatus

Genetic indices and statistics	Haplogroup A							Haplogroup B	Overall data set
	DRC			Burundi		Rwanda AEZ2	Haplogroup A (overall)
	AEZ1	AEZ2	AEZ3	AEZ1	AEZ3
Diversity indices
Number of sequences	39	50	44	25	17	14	189	20	209
Number of polymorphic sites	7	9	6	8	4	7	18	2	27
Number of haplotypes	8	9	6	7	5	6	19	3	22
Haplotype diversity (SD)	0.76 (0.044)	0.78 (0.032)	0.77 (0.033)	0.7 (0.084)	0.77 (0.057)	0.77 (0.089)	0.77 (0.016)	0.56 (0.063)	0.81 (0.01)
Nucleotide diversity (SD)	0.002 (0.0003)	0.0025 (0.0003)	0.002 (0.0003)	0.002 (0.0004)	0.002 (0.0003)	0.002 (0.0006)	0.002 (0.0001)	0.001 (0.0002)	0.006 (0.0006)
Neutrality tests
Tajima's D (P-value)	-0.6 (0.35)	-0.79 (0.23)	0.096 (0.6)	-1.4 (0.049)*	-0.21 (0.45)	-1.4 (0.07)	-1.5 (0.032)*	0.24 (0.67)	-0.93 (0.23)
Fu's Fs (P-value)	-2.4 (0.08)	-2.5 (0.097)	-0.1 (0.49)	-2.6 (0.027)*	-1.1 (0.18)	-2.1 (0.041)*	-10.4 (0.001)*	0.2 (0.49)	-3.8 (0.15)
Demographic expansion
Sum of Squared deviation (P-value)	0.002 (0.66)	0.004 (0.31)	0.002 (0.49)	0.002 (0.72)	0.02 (0.17)	0.001 (0.9)	0.0008 (0.44)	0.029 (0.049)*	0.017 (0.1)
Harpending’s Raggedness index (P-value)	0.06 (0.39)	0.079 (0.21)	0.06 (0.38)	0.05 (0.71)	0.16 (0.12)	0.06 (0.73)	0.057 (0.2)	0.21 (0.1)	0.049 (0.51)
Spatial expansion
Sum of Squared deviation (P-value)	0.002 (0.5)	0.004 (0.21)	0.002 (0.4)	0.002 (0.67)	0.02 (0.1)	0.001 (0.9)	0.0008 (0.26)	0.029 (0.011)*	0.036 (0.28)
Harpending’s Raggedness index (P-value)	0.064 (0.39)	0.079 (0.21)	0.062 (0.43)	0.05 (0.75)	0.16 (0.12)	0.06 (0.72)	0.057 (0.22)	0.21 (0.12)	0.049 (0.74)

Abbreviations: D Tajima’s neutrality statistic, Fs Fu’s neutrality statistic

*Values are statistically significant at P < 0.05; significance was determined using 1000 coalescent simulations