Fig. 5.

Silencing of STAT1 protein expression by siRNA attenuates the MG132-induced cell apoptosis. a. By Western blot analysis, we detected the expression of cleavage and total caspase 3 and PARP in EC1 and KYSE150 in a time- and dose-dependent MG132 treatment manner. b. Cell death induced by 1–5 μM MG132 was detected by MTS assay in both EC1 and KYSE150. c. MTS assay was used to detect the 5 μM MG132-induced cell death in both EC1 and KYSE150 cell lines with STAT1 siRNA and scrambled RNA (Scr RNA) transfection. d. KYSE150 cells were transfected with STAT1 siRNA or scrambled RNA and treated with 5 μM MG132 or control. The cleavage and total expression of PARP and caspase 3 was detected by using Western blot analysis. e. Apoptosis was detected by flow cytometry in KYSE150 cells transfected with STAT1 siRNA or scramble RNA. Values are expressed as means ± SD of three independent experiments. (*p < 0.05)