Table 1.

Recipes for Buffers used for the Protocol

Components	Volume
50X TAE Buffer (500 ml)
Tris Buffer GR	121 g
EDTA Stock (0.5 M)	50 ml
Glacial Acetic Acid	28.55 ml
5× TBE Buffer (1 l, pH 8.3)
Tris Buffer GR	54 g
Boric Acid	27.5 g
EDTA Stock (0.5 M)	20 ml
30% Acrylamide (25 ml)
Acrylamide	7.3 g
Bis-acrylamide	0.2 g
8% Polyacrylamide Gel
30% Acrylamide	2.620 ml
5x TBE Buffer	1.97 ml
Autoclaved Milli Q water	5.24 ml
10% Ammonium Persulfate	163 μl
TEMED	8 μl
Total	10 ml
EtBr (for staining)	0.5 μg/ml in 1X TBE
10X TE Buffer (pH 8.0)
Tris Buffer	1 ml (Stock 1 M; Final concentration 100 mM)
EDTA	200 μl (Stock 1 M; Final concentration 10 mM)
Autoclaved Milli Q water	8.8 ml