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. 2018 Jun 1;16:61. doi: 10.1186/s12915-018-0523-6

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© Hidalgo et al. 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Kinetics of Tpx1, Trx1, Pap1, and HA-OxyR in wild-type cells upon sub-toxic and toxic concentrations of H₂O₂. a Cultures of strain AD29 (WT expressing HA-OxyR) were treated or not with 0.1 mM (left panels) or 0.5 mM (right panels) H₂O₂ for the indicated times, and protein extracts were obtained and processed as described in Fig. 1b, using antibodies against Tpx1 (ox. Tpx1 dimer and red. Tpx1 monomer), sulfinylated Tpx1 (Tpx1-SO₂H), Trx1 (red. Trx1 and ox. Trx1), Pap1 (red. Pap1 and ox. Pap1), or HA (red. HA-OxyR and ox. HA-OxyR). b ,c Average of biological triplicates (b) or duplicates (c) of the experiment described in (a) are represented in curve graphs. Error bars (standard error of the mean) from the triplicates are shown. ox. oxidized, red. reduced, WT wild type