Fig. 3.

AtPep3/AT13 peptide induced salinity stress tolerance. (A) Full-length amino acid sequence of AT13 and synthetic peptide fragments used in the salt treatment assay. Overlapping peptide fragments covering the entire AT13 protein (∼30 amino acids in length) were synthesized. Sequence details are provided in SI Appendix, Table S3. (B and C) Images of representative plants after each peptide/salt treatment and chlorophyll content per fresh weight (FW) in plants after each peptide/salt treatment, respectively. Seven-day-old plants were pretreated with 10 µM of a peptide fragment for 3 d (peptide pretreatment) followed by a combined treatment with the peptide plus 150 mM NaCl. All treatments were carried out in liquid culture. Plants were grown for 10 d and chlorophyll content was subsequently assessed.