Fig. 4.

Erβ stimulation reduces the size of the area depleted of neurons by benzalkonium chloride (BAC). Adult male C57BL/6 mice were treated with topical administration of BAC on the ileal serosa. After 4 d, mice were s.c. implanted with slow-releasing pellets containing LY3201 or vehicle. Both groups were killed at day 11 after BAC treatment and the size of the area (in square millimeters) depleted of neurons was assessed via immunofluorescence with anti-HuC/D staining. (A and B) Immunofluorescence images of muscularis externa stained for HuC/D from vehicle-treated (A) and LY3201-treated (B) mice. (C) Points represent the area depleted of neurons (in square millimeters) from individual mice. Data are representative of two independent experiments. Nonparametric Mann–Whitney test (***P < 0.0001).