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. 2018 May 14;115(22):5768–5773. doi: 10.1073/pnas.1721650115

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Fig. 1. — Autophagy is essential for survival of GSCs under anoikis conditions. (A) Percentage of autophagy in anoikis-resistant GSCs determined by flow cytometry. Fluorescence and phase-contrast images captured of the same cells [autophagic vacuoles (green) and blebbing/punctae]. (Magnification: 60×.) (B) Live/dead assay of neurospheres and GSC viability under conditions of autophagy induction (rapamycin treatment) and inhibition (CQ treatment) (green cells are viable). (Magnification: 100×.) Confocal images quantified for cell death and graphically represented. Error bars indicate ±SD, *P < 0.05.