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EGFR activation regulates protective autophagy in anoikis-resistant GSCs. Constitutive activation of EGFR decreases autophagy. (A) Effect of constitutive EGFR activation on EGFR, PKC, BCL2 signaling, and MDA-9, LC3B expression by Western blotting. (B) Effect of constitutive EGFR activation on EGFR, pEGFR, ATG5, Lamp1, and LC3B expression determined by flow cytometry. Error bars indicate ±SD, *P < 0.05.
