Fig. 6.

CD9 ATMs are present in CLSs of human adipose and correlate with BMI. (A) Flow-cytometric analysis of VAT ATMs (gating strategy in SI Appendix, Fig. S6). (B) Surface marker expression and intracellular lipid levels of VAT ATMs (normalized to percentage mode of MFI; Neg., Lymphocyte gate; statistical comparison between CD9⁻ and CD9⁺ ATMs by ratio paired Student’s t test, *P < 0.05, **P < 0.01; n = 8–12). (C) Immunohistochemistry of VAT from obese individuals. Sections stained for DAPI, CD11b, and CD9. (D) Representative imaging flow cytometry of VAT CD9⁻ or CD9⁺ ATMs. (Scale bars: C, 50 μm; D, 10 μm.) (E) Correlation between CD9⁺ ATM number and BMI (analysis by linear regression, n = 12).