FIG. 8.

Blood vessel morphological assessment by immunofluorescence staining. Representative DAPI (blue), CD31 (green), and αSMA (red) co-staining of 8-week cross-sections (region I) for the groups: (A) S, (B) S + ECM, and (C) S + ECM + D. (D) Blood vessel number and type quantification, n ≥ 5, *p < 0.05 for total blood vessels. Arrows in (A–C) indicate blood vessels. Details on the adopted algorithm for vessel segmentation are provided in Supplementary Fig. S1. DAPI, 4′,6-diamidino-2-phenylindole. Color images available online at www.liebertpub.com/tea