FIG. 5.

Construct morphology and bone protein deposition. Histology and immunohistochemistry of constructs for general histomorphology and bone-specific proteins: Movat's pentachrome for GAG (green), collagen (yellow), decellularized scaffold (dark yellow), and cell nuclei (red/black). Immunohistochemistry for BSP and osteopontin. Magnified images are shown for both the edge and center of the construct. Scale bars: 50 μm. BSP, bone sialoprotein. Color images available online at www.liebertpub.com/tea