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. Author manuscript; available in PMC: 2018 Jun 2.
Published in final edited form as: Stem Cells. 2016 Sep 20;35(2):351–361. doi: 10.1002/stem.2484

Figure 1. Characterization of VSMCs differentiated from ESCs derived CVPCs: highly efficient and comparable with primary human VSMCs.

Figure 1

(A): Strategy of VSMC differentiation from CVPCs. (B): Flow cytometry analysis of SMMHC positive cells on CVPC and day7 VSMC. (C): Compare expression of contractile markers between CVPC-VSMC, primary human VSMC and primary human fibroblast by RT-qPCR. (D): Compare expression of contractile markers and cell morphology between CVPC-VSMC and primary human VSMC by immunofluorescence with low magnification (Left, scale bar=100 μm) and high magnification (Right, scale bar=20 μm). RT-qPCR was shown as average ± SEM (n=3). TGF-β: transforming growth factor β; CVPC: cardiovascular progenitor cell; VSMC: vascular smooth muscle cell; SMMHC: smooth muscle myosin heavy chain.