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. Author manuscript; available in PMC: 2018 Jun 2.
Published in final edited form as: Stem Cells. 2016 Sep 20;35(2):351–361. doi: 10.1002/stem.2484

Figure 6. YAP interfered with NKX2.5 protein during CVPC-VSMC differentiation and attenuates VSMC differentiation in a NKX2.5 dependent manner.

Figure 6

(A): Chip-PCR assays between Vector control and YAP overexpressed groups were confirmed in CVPC-derived VSMCs. (B): CO-IP assay on CVPC-derived VSMCs to detect protein-protein interaction between overexpressed YAP and endogenous NKX2.5. (C): Knockdown of NKX2.5 during CVPC-VSMC differentiation by different shRNAs: shNKX2.5-1 and shNKX2.5-2 while sh-scramble was used as control and MYOCD and VSMC markers were detected by RT-qPCR. (D): Knockdown of NKX2.5 abolished effect of YAP+TAZ knockdown during CVPC-VSMC differentiation. Results were shown as average ± SEM and statistic analyzed with unpaired t-test. *p<0.05, **p<0.01. IP: immunoprecipitation; IB: immunoblot. M: molecular mass markers.