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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Clin Cancer Res. 2018 Mar 14;24(11):2642–2652. doi: 10.1158/1078-0432.CCR-17-2778

Figure 3.

Figure 3

(A) Photograph of gradient separation in U87 cells with arrow indicating low density lipid raft band corresponding to survivin-containing fraction #4. Western blot detection of lipid raft-associated proteins and survivin in subcellular fractions derived from U87 (B) and A1207 (C) glioblastoma cells. (D) Transiently expressed recombinant c-Myc-tagged human survivin in association with lipid raft protein fractions in HEK293T cells detected with an anti-c-Myc-tag antibody (9E10). (E) Imagestream imaging flow cytometric visualization of co-localized bound cholera toxin B (CT-B) and survivin in lipid rafts.