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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Cancer Res. 2018 Mar 9;78(11):2897–2910. doi: 10.1158/0008-5472.CAN-17-3531

Figure 2. Mapping of interaction regions between Mdmx and Peli1.

Figure 2

(A) A schematic representation of Mdmx and the corresponding mutants (+: binding positive to Peli1; -: binding negative to Peli1).

(B) FH-Mdmx and the corresponding deletion mutants were individually transfected with Myc-Peli1 in H1299 cells. Whole cell lysates and immunoprecipitates with FLAG/M2 beads were subjected to western blot with anti-Myc (top) and anti-HA (lower) monoclonal antibodies.

(C) Myc-Mdmx and the Myc-Mdmx Δ200–274 mutant were individually transfected with FH-Peli1 in H1299 cells. Whole cell lysates and immunoprecipitates with anti-Myc antibody were subjected to western blot with anti-HA (top) and anti-Myc (lower) monoclonal antibodies.

(D) A schematic representation of Peli1 and the corresponding mutants (+: binding positive to Mdmx; -: binding negative to Mdmx).

(E) Myc-Peli1 and the corresponding mutants were individually transfected with FH-Mdmx in H1299 cells. Whole cell lysates and immunoprecipitates with FLAG/M2 beads were subjected to western blot with anti-HA (top) and anti-Myc (lower) monoclonal antibodies.