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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Cancer Res. 2018 Mar 20;78(11):3075–3086. doi: 10.1158/0008-5472.CAN-17-3024

Figure 5. Role of BAX, BAK, and BIM in venetoclax/GDC-0980 lethality in venetoclax-sensitive MV4-11 cells.

Figure 5

Cells in which BAX, BAK, double BAX/BAK (A–B), or BIM (C–D) were knocked out using CRISPR or non-targeting (NT) control cells were exposed to 10 nM venetoclax and/or 500 nM GDC-0980. Cells were either lysed after 2 hr and subjected to western blot analysis (A–C), or analyzed for the extent of apoptosis at 5 hr using Annexin V staining (B–D). Error Bars, S.D for at least 4 independent experiments. For B: P = 0.0004; P = 0.0027; P = 0.0002 for BAX−/−, BAK−/−, or BAX−/−BAK−/− cells respectively compared to NT cells; for D: P = 0.1307 for BIM−/− cells vs NT cells.