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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Cancer Res. 2018 Mar 23;78(11):2852–2863. doi: 10.1158/0008-5472.CAN-17-2992

Figure 5. IGF-1R and MDA-9/Syntenin physically interact and regulate STAT3 activity.

Figure 5

A) RWPE-1 cells treated with hIGFBP-2 under different conditions and phospho-IGF-1R expression determined by Western blotting. B) 200 µg of total protein from ARCaP-M cells incubated with MDA-9/Syntenin overnight for immunoprecipitation and Western blotting performed with anti-IGF-1R antibody. C) Immunofluorescence assay to determine co-localization of proteins. Confocal microscopy images of MDA-9/Syntenin and IGF-1R as “separate” or “merged” images. D) RWPE-1 cells transfected with wild type or mutant mda-9 vectors. 48 hours later, cells replated on fibronectin-coated plates for 30 mins and cell lysates analyzed using the indicated antibodies. E) PCa cell lines infected with Ad.5/3-shcon or Ad.5/3-shmda-9 for 48 hours. Cells reseeded on fibronectin-coated plates and treated with recombinant hIGFBP-2 (100 ng/ml) for 1 hour. Western blotting analysis to determine auto-phosphorylation.