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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Cancer Res. 2018 Mar 23;78(11):2852–2863. doi: 10.1158/0008-5472.CAN-17-2992

Figure 6. MMP-2 and MMP-9 are key regulators of MDA-9/Syntenin-mediated PCa invasion.

Figure 6

A) PCa cell lines infected with Ad.5/3-shcon or Ad.5/3-shmda-9 for 48 hours at 100 pfu/cell. Cells reseeded on fibronectin-coated plates and treated with recombinant hIGFBP-2 (100 ng/ml) for 1 hour. Western blotting to determine MMP-2 and MMP-9 expression. B) Cells were co-transfected/infected with different adenovirus/plasmids, followed by 48 hours incubation for transgene expression. Western blotting performed using anti-MMP-2 and anti-MMP-9. C) Cells were co-transfected with different expression plasmids. 48 hours later, cells were trypsinized and invasion determined. Average with S.D. from three independent experiments. “*” represents statistical significance (p<0.05) between indicated groups.