Table 4.
Comparison of liquid chromatographic tendon mass spectrometry methods to quantify CUR
| Current method |
Chen, Z. et. al.[16] |
Ratnatilaka Na Bhuket, P. et. al.[19] |
Ma, W. et. al.[20] |
Ramalingam, P. et. al.[21] |
Cao, Y. et. al.[15] |
|
|---|---|---|---|---|---|---|
| Separation column | Agilent Zorbax Eclipse XDB C18 column (3.5 μm, 4.6×50 mm) | Agilent Poroshell SB-C18 (2.7 μm, 4.6 ×150mm) | Halo C8 column (2.7 μm, 4.6 × 50mm) | Waters ACQUITY UPLC BEH C18(1.7 μm, 100 × 2.1mm) | Sepax BR-C18 (5 μm, 1.0 × 100 mm) | Thermo BetaBasic-8 column (5 μm, 2.1 × 50 mm) |
|
| ||||||
| Detection Instrumentation | Thermo Finnigan LTQ XL iontrap MS, CRM | Thermo TSQ Quantum, SRM | AB SCIEX QTRAP®6500 mass spectrometer, MRM | AB SCIEX QTRAP®5500 tandem mass spectrometer, MRM | 6490 triple quadrupole MS, MRM | API 3000 quadrupole mass, MRM |
|
| ||||||
| Ionization/Mass Spectrometry mode | ESI/MS3 with Ion Trap MS | ESI/MS2 with Quadrupole | ESI/MS2 with Triple-Quadrupole | ESI/MS2 with Quadrupole | ESI/MS2 with Quadrupole | ESI/MS2 with Quadrupole |
|
| ||||||
| Ion pairs for CUR quantification | 369-245-213 | 369-179 | 369-177 | 369-177 | 369-285 | 369-177 |
|
| ||||||
| Internal Standard Type | CUR-d6 | Verapamil | dimethylcurcumin CUR analog | Glibenclamide CUR structure similarities | Salbutamol | Hesperetin |
| Deuterium labeled CUR | CUR structure similarities | CUR structure similarities | CUR structure similaities | |||
|
| ||||||
| Sample preparation method | Two dimension | Single dimension | Single dimension | Single dimension | Single dimension | Single dimension |
| PPE with acetonitrile and SEC | LLE with methyl tert-butyl | PPE with acetonitrile | PPE with methanol | LLE with ethyl acetate | LLE with ethyl acetate | |
|
| ||||||
| Recovery rate (%) | 96.2–109.3 | 90.3–102.8 | 81.4–86.7 | 89.2–100.5 | 77.7–84.1 | 35.2–44.6 |
|
| ||||||
| Recovery RSD (%) | 1.3–2.2 | 8.2–10.7 | 9.6–14.0 | 9.6 | 4.6–13.8* | N/A |
|
| ||||||
| Matrix Effect (%) | 95.4–111.0 | 76.7–88.6 | 41.4–44.3 | 89.2–105.9 | 101.9–103.5 | 69.2–80.3 |
|
| ||||||
| ME RSD (%) | 1.3–8.1 | 7.4–11.4 | N/A | N/A | 2.3–4.2* | N/A |
|
| ||||||
| LLOQ (ng/ml) | 1 | 1 | 1 | 5 | 2.5 | 2 |
|
| ||||||
| LLOD (ng/ml) | 0.1 | 0.5 | N/A | N/A | N/A | 1 |
|
| ||||||
| Linear Calibration range:ng/ml | 1–3000 | 1–1000 | 1–500 | 5–2000 | 2.5–2000 | 2–500 |
|
| ||||||
| Retention time (min) | 2.5 | 1.5 | 1.5 | 4.1 | 2.2 | N/A |
|
| ||||||
| Intra-assay accuracy (%) | −5.1–3.2 | 0.9–13.8 | −4.8–8.5 | −2.1–6.1 | −1.3–4.7 | −1.3–8.0 |
|
| ||||||
| Inter-assay accuracy (%) | −1.8–1.6 | −0.7–9.1 | −3.3–1.5 | −2.0–4.6 | −6.6–0.7 | −2.1–4.0 |
|
| ||||||
| Intra-assay precision (RSD %) | 1.9–4.4 | 2.1–5.1 | 3.1–12.6 | 7.7–14.4 | 1.8–12.3 | 4.7–7.6 |
|
| ||||||
| Inter-assay precision (RSD %) | 2.0–5.1 | 6.2–10.3 | 8.8–10.7 | 0.6–5.6 | 1.7–13.1 | 1.8–10.9 |
|
| ||||||
| Remarks | First ITMS3; PPE+SEC; Curcumin-d6; 1–3000 ng/ml for curcumin Linear calibration range | UPLC-MS-MS | protein precipitation method | 5–2000 ng/ml for curcumin Linear calibration range | LC-MS/MS method for the analysis of curcumin in mouse plasma and brain tissue | 2–1000 ng/ml for curcumin Linear calibration range |
*
calculated by (Standard Deviation/Average) ×100%