(A) Schematic of organoid plate production.
(B) Representative wells of 96-well and 384-well kidney organoid plates at identical magnification, showing phase contrast image with proximal tubule (LTL) overlay in green. Zoom of boxed region (arrowhead) is shown below.
(C) Representative wells of a 384-well organoid plate labeled with nephron segment markers of proximal tubule (LTL), distal tubule (ECAD), and podocytes (NPHS1), showing progressive zoom of yellow boxed regions.
Scale bars, 100 µm. See also related Figure S1.