KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Fluorescein Lotus tetragonolobus Lectin	Vector Labs	FL-1321; RRID:AB_2336559
Rat Anti-Human E-Cadherin antibody [DECMA-1]	Abcam	Ab11512; RRID:AB_298118
Sheep Anti-Human Nephrin (NPHS1)	R&D Systems	AF4269; RRID:AB_2154851
Mouse Anti-Human ZO-1 antibody	Invitrogen	339100; RRID:AB_2533147
Mouse Anti-Human CD31	BD BIosciences	555444; RRID:AB_395837
Bacterial and Virus Strains
Biological Samples
Developing kidney tissue (days 60–130)	Laboratory of Developmental Biology (University of Washington)	26805, 26846, 26847, 26848
Chemicals, Peptides, and Recombinant Proteins
GSK-3β inhibitor CHIR99021	StemGent	04-0004-10; CAS 252917-06-9
Rho-kinase inhibitor Y27632	StemGent	04-0012; CAS 146986-50-7
Cisplatin	Sigma	1134357; CAS 15663-21-1
Forskolin	LC Laboratories	FF-9929; CAS 66575-29-9
Recombinant human VEGF165	Peprotech	100–20
Critical Commercial Assays
CellTiter-Glo	Promega	G75070
Kidney Injury Panel 3 Human Kit	Meso Scale Diagnostics	K15189D-1
Deposited Data
scRNA-seq samples	This paper	GEO: GSE109718
Human Developing Kidney scRNA-seq	Menon et al., 2018	GEO: GSE109205
CHIR99021 titration in 384-well plates of human pluripotent stem cells – analysis of nephron segment differentiation	This paper	Mendeley Data, doi:10.17632/988tyf4fh8.1
Experimental Models: Cell Lines
WA09 (H9) human ES cells	WiCell	WAe009-A
WTC11 human iPS cells	Laboratory of Bruce Conklin, MD	GM25256; RRID:CVCL_Y803
CRISPR-mutant PKD hPSC and isogenic controls	Freedman et al., 2015 Cruz et al., 2017	PKD1−/− A3, F1, C6 PKD2−/− G4, 12 control 07, B4, B5, H1, H7
Experimental Models: Organisms/Strains
NOD-scid mice (NOD.CB17-Prkdcscid/J)	Jackson Laboratory	RRID:IMSR_JAX:001303
Oligonucleotides
Recombinant DNA
Software and Algorithms
GE INCELL Investigator/Developer	GE	http://gelifesciences.com
GraphPad Prism	GraphPad	http://graphpad.com
NIS Elements	Nikon	http://nikoninstruments.com
ImageJ	NIH	http://imagej.nih.gov
Other