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. 2018 Jun 1;18:621. doi: 10.1186/s12885-018-4326-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Molecular mechanism associated with stable Slit3 repression. Protein expression of certain proteins associated with cell cycle progression (cyclin D3), apoptosis (survivin) and GSK3β/β-catenin pathway (β-catenin and phosopho-GSK3β (ser9) in LM3 and PLC Slit3 shRNA transfectants (shSlit3) and shRNA control (shCTL). The protein expression level was quantified by ImageJ software and normalized to the expression of actin. The experiment was repeated for three times and one representative blot was shown