Skip to main content
. 2018 Jun 1;17:219. doi: 10.1186/s12936-018-2369-3

Table 1.

Antigens included in the multiplex qSAT panel

 Antigens and genotype Life-cycle stage Rationale References
Pre-erythrocytic (PE)
 CelTOS Sporozoite Exposure to sporozoite [31, 32]
 CSP full length* Sporozoite Exposure to sporozoite and RTS,S specific [30, 33]
 CSP NANP repeat* GST-fused Sporozoite Exposure to sporozoite and RTS,S specific [35]
 CSP C-terminus* GST-fused Sporozoite Exposure to sporozoite [36]
 SSP2 or TRAP Sporozoite Representative of exposure to sporozoite [34, 37]
Liver stage
 LSA-1* Liver stage Liver stage antigen—infected hepatocytes [38, 39]
Blood stage (BS)
 AMA-1 3D7 (FMP2.1)* Merozoite Involved in erythrocyte invasion [40, 41]
 AMA-1 FVO (FMP009) Merozoite Involved in erythrocyte invasion [41]
 CyRPA full length Merozoite Involved in erythrocyte invasion [42]
 EBA-140 GST-fused Merozoite Involved in erythrocyte invasion [43]
 EBA-175 R2 PfF2 Merozoite Involved in erythrocyte invasion [44]
 EBA-175 R3–5* GST-fused Merozoite Involved in erythrocyte invasion [43]
 EXP-1 Merozoite Involved in erythrocyte invasion [45]
 MSP-1 Block 2 3D7* GST-fused Merozoite Involved in erythrocyte invasion [46]
 MSP-1 Block 2 hybrid GST-fused Merozoite Involved in erythrocyte invasion [47]
 MSP-1 Block 2 MAD20 GST-fused Merozoite Involved in erythrocyte invasion [46]
 MSP-1 Block 2 PA17 GST-fused Merozoite Involved in erythrocyte invasion [46]
 MSP-1 Block 2 RO33 GST-fused Merozoite Involved in erythrocyte invasion [46]
 MSP-1 Block 2 Well GST-fused Merozoite Involved in erythrocyte invasion [46]
 MSP-142 3D7* Merozoite Involved in erythrocyte invasion [41, 48]
 MSP-142 FVO Merozoite Involved in erythrocyte invasion [41, 48]
 MSP-2 full length B* GST-fused Merozoite Representative of exposure to BS [49]
 MSP-2 full length A* GST-fused Merozoite Representative of exposure to BS [49]
 MSP-3 3C Merozoite Representative of exposure to BS [49]
 MSP-3 3D7* Merozoite Representative of exposure to BS [50]
 MSP-5 Merozoite Representative of exposure to BS [51, 52]
 MSP-6* GST-fused Merozoite Representative of exposure to BS [53]
 P41 Merozoite Involved in erythrocyte invasion [54]
 RH1 Merozoite Involved in erythrocyte invasion [55]
 RH2 (2030) GST-fused Merozoite Involved in erythrocyte invasion [56]
 RH2 b240 Merozoite Involved in erythrocyte invasion [57]
 RH4.2 GST-fused Merozoite Involved in erythrocyte invasion [58, 59]
 RH4.9* Merozoite Involved in erythrocyte invasion [58, 59]
 RH5 Merozoite Involved in erythrocyte invasion [42, 60]
 PTRAMP Merozoite Involved in erythrocyte invasion [61]
 DBL-α Trophozoite Involved in cytoadherence [62]
Pregnancy-specific
 DBL1-DBL2 VAR2CSA Trophozoite Associated to placental malaria exposure and representative of maternally-transferred antibodies [63]
 DBL3-DBL4 VAR2CSA* Trophozoite [64]
Other antigens
 HBsAg* NA Hepatitis B surface antigen
 α-Gal Involved in malaria protection [30]
Controls
 GST* Background Control fusion protein
 BSA* Background Control unspecific binding

* Recombinant proteins used for the experimental assessment of the optimal temperature and time of samples incubation in the IgG assays. MSP-2 A corresponds to the CH150 strain and MSP-2 B to the Dd2 strain