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. 2018 Jun 1;19:425. doi: 10.1186/s12864-018-4820-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Developmental stage-dependent genomic associations and TE expression modulation of piRNA candidates. a Genomic association varied in stage-dependent fashion. Each feature was calculated in proportion to the respective total piRNA candidates. b PiRNA candidates mapped to TEs. piRPM is calculated for each enlisted category. c The stage-dependent association of piRNAs to subfamilies of LINEs. PiRNA sequences are preferentially mapped antisense to TEs. d Expression of CR1 subfamily members from enriched embryonic germ cells. e Stranded RT-qPCR analysis (N = 3) over CR1-C transcription among cultured E3 circulating PGC (E3PGC), cultured E7 gonadal PGC (E7PGC), and germ cell enriched population, E11Germ and E14Germ, from E11 and E14 Gonads, respectively. ** represents p-value < 0.01