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. 2018 Jun 1;19:425. doi: 10.1186/s12864-018-4820-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — PiRNA cluster analysis reveals stage-dependent differential expression of piRNA precursors. a PiRNA cluster locus identified in each sample via proTRAC. Each bar represents a cluster at the plus (up) or minus (down) strand. Length and color depth denote the piRNA density in percentile. b Multidimensional scaling reveals the stage-associated piRNA cluster expression profile. Eigenvalues approximate the degree of variation explained by each dimension. c Identification of stage-enriched piRNA clusters (piRCs), each of which has piRPKM 1.5-fold higher than the second-highest stage. Stage-enriched piRNA clusters from blastodermal cells (BC), primordial germ cells (PGC), embryonic gonads from E11 and E14 (EG), and adult testes (AT) are included