Fig. 6.

Hippocampal neurons have impaired activation toward acute stress in NL2 R215H KI mice. a Typical appearance of WT and KI mice after restraining. b Quantified corticosteroid level of WT and KI mice at the baseline level and after 1 h restraining. 4 to 8 mice were used for each genotype at each condition, age 4 to 6 months. Two-way ANOVA with Sidak’s multiple comparison test was used for analysis. c Upper row: representative images of baseline cFos immunoreactivity of WT and R215H KI hippocampus; bottom row: cFos immunoreactivity of WT and R215H KI hippocampus after restraining. d enlarged DG and CA2/3 region of WT and R215H mice after restraining. e Quantification of cFos-positive cells at DG granule cell layers. f Quantification of cFos positive cells at CA2/3 pyramidal cell layers. WT: n = 16 brain slices / 4 mice; R215H KI: n = 20 brain slices / 5 mice; scale bar = 200 μm. Student’s t-test was used for analysis. Data represent mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001