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qRT-PCR analyses of 20 significantly changed piRNAs to validate RNA-Seq. a Comparison of the 20 most significantly changed piRNA expression levels among obstructive azoospermia (OA) groups, USR groups, and SSR groups by qRT-PCR. b qRT-PCR analyses of 20 significantly changed piRNA expression levels in human seminal plasma and testicular tissues. Data are shown as mean ± SEM (n = 5)
