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Confocal images of oocytes at 18 h after ICSI, showing presence of a protrusion from the oocyte (“extrusion cone,” arrow) in the area of a polar body (arrowhead). The extrusion cone was associated with presence of cytoplasmic extrusions in the perivitelline space (b, c). The cytoplasmic extrusions tended to have accumulations of PNA-positive granules (c)
